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Article

A mutational study of the site-specific cleavage of EC83, a multicopy single-stranded DNA (msDNA): nucleotides at the msDNA stem are important for its cleavage.

Journal of bacteriology - 1 Oct 1997

Kim K, Jeong D, Lim D

Abstract excerpt

Multicopy single-stranded DNA (msDNA) molecules consist of single-stranded DNA covalently linked to RNA. Such molecules are encoded by genetic elements called retrons. Unlike other retrons, retron EC83 isolated from Escherichia coli 161 produces RNA-free msDNA by site-specific cleavage of msDNA at 5'-TTGA/A-3', where the slash indicates the cleavage site. In order to investigate factors responsible for the msDNA...

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Identifiers

PMID: 9335306
DOI: 10.1128/jb.179.20.6518-6521.1997
PMCID: PMC179573

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