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Article

Asymmetric DNA methylation by dimeric EcoP15I DNA methyltransferase.

Biochimie - 1 Jan 2000

Urulangodi Madhusoodanan, Dhanaraju Rajkumar, Gupta Kanchan, Roy Rajendra P, Bujnicki Janusz M, Rao Desirazu N

Abstract excerpt

EcoP15I DNA methyltransferase (M.EcoP15I) recognizes short asymmetric sequence, 5'-CAGCAG-3', and methylates the second adenine only on one strand of the double-stranded DNA (dsDNA). In vivo, this methylation is sufficient to protect the host DNA from cleavage by the cognate restriction endonuclease, R.EcoP15I, because of the stringent cleavage specificity requirements. Biochemical and structural characterization...

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Identifiers

PMID: 27422119
DOI: 10.1016/j.biochi.2016.07.006

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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