Article
A comparative study of HLA-DRB typing by transcription-mediated amplification with the hybridization protection assay (TMA/HPA) versus PCR/SSOP.
Human immunology - 1 Jun 1997
Smith A G, Matsubara K, Mickelson E, Marashi A, Regen L, Guthrie L A, Hansen J A
Abstract excerpt
To evaluate alternative human leukocyte antigen (HLA)-DNA typing methods, we used a system of transcription-mediated amplification (TMA) with a probe hybridization protection assay (HPA) in a microtiter plate format developed by Chugai Pharmaceutics Ltd. (Tokyo, Japan) to perform intermediate-lev...
Topics
- Alleles
- Amino Acid Sequence
- Gene Amplification
- HLA-DR Antigens
- HLA-DRB1 Chains
- HLA-DRB3 Chains
- HLA-DRB4 Chains
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization