Article
A comparative study of HLA-DRB1 typing by standard serology and hybridization of non-radioactive sequence-specific oligonucleotide probes to PCR-amplified DNA.
Tissue antigens - 1 Feb 1993
Mickelson E, Smith A, McKinney S, Anderson G, Hansen J A
Abstract excerpt
A double-blind study was carried out to evaluate the relative performance and reliability of the PCR/SSOP assay compared to conventional serological typing in identifying HLA-DR alleles. A total of 268 consecutive samples were entered into the study. In 14 (5.2%) of the cases, HLA-DR serology cou...
Topics
- Alleles
- Base Sequence
- Double-Blind Method
- Evaluation Studies as Topic
- HLA-DR Antigens
- HLA-DRB1 Chains
- Histocompatibility Antigens Class II
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Serology