Article
Probe-less genomic typing of Arg52 (type 1 diabetes-associated) and non-Arg52 (non-type 1 diabetes-associated) HLA-DQA1 alleles.
Autoimmunity - 1 Jan 1992
Sorrentino R, Costanzi S, Cascino I, Tosi R
Abstract excerpt
According to recent evidence, the presence of an Arg residue at position 52 in the HLA-DQ alpha chain may confer susceptibility to Type 1 diabetes and thus be possibly used to define quantitatively the genetic risk of this disease. Arg52 and non-Arg52 DQA1 alleles cannot be typed by the conventional cytotoxicity test and they must be distinguished at the genomic level. We describe a simple procedure which...
Topics
- Alleles
- Cell Line
- DNA
- Diabetes Mellitus, Type 1
- Disease Susceptibility
- Electrophoresis, Polyacrylamide Gel
- Electrophoresis, Starch Gel
- Genome
- Genotype
- HLA-DQ Antigens
- HLA-DQ alpha-Chains