Article
A yeast sterol auxotroph (erg25) is rescued by addition of azole antifungals and reduced levels of heme.
Proceedings of the National Academy of Sciences of the United States of America - 14 Oct 1997
Gachotte D, Pierson C A, Lees N D, Barbuch R, Koegel C, Bard M
Abstract excerpt
Genetic disruption of the Saccharomyces cerevisiae C-4 sterol methyl oxidase ERG25 gene leads to sterol auxotrophy. We have characterized a suppression system that requires two mutations to restore viability to this disrupted strain. One suppressor mutation is erg11, which is blocked in 14alpha-d...
Topics
- Antifungal Agents
- Azoles
- Clotrimazole
- Genes, Fungal
- Genotype
- Heme
- Itraconazole
- Ketoconazole
- Lanosterol
- Mixed Function Oxygenases
- Mutation
- Saccharomyces cerevisiae
- Sterols
- Suppression, Genetic