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Article

Engraftment of normal stem cells in W/Wv mice assessed by a novel quantitative PCR analysis.

British journal of haematology - 1 Sept 1997

Tanosaki R, Migliaccio A R

Abstract excerpt

We describe a quantitative PCR based on the unique Nsi 1 restriction site generated by the Wv mutation (C to T substitution at position 2007 of c-kit) to quantify the percentage of engraftment of wild-type stem cells in W/Wv mice. Primers flanking this mutation specifically amplify c-kit sequences from either genomic DNA or cDNA. After Nsi 1 digestion, wild-type products were identified by electrophoresis as a...

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Identifiers

PMID: 9326206
DOI: 10.1046/j.1365-2141.1997.2983115.x

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