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Quantitative determination of bone marrow transplant engraftment using fluorescent polymerase chain reaction primers for human identity markers

1 Apr 1995

Abstract excerpt

We have developed a quantitative, nonisotopic method using variable number tandem repeat (VNTR) and short tandem repeat (STR) markers for monitoring donor cell engraftment in marrow transplant recipients. Posttransplant DNA from the recipient is amplified with fluorescent polymerase chain reaction (PCR) primers for polymorphic markers that distinguish donor alleles from recipient alleles. The fluorescent PCR...

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PMID: 7703498
DOI: 10.1182/blood.v85.7.1954.bloodjournal8571954

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