Article
Characterization of human endothelin B receptor and mutant receptors expressed in insect cells.
European journal of biochemistry - 15 Aug 1997
Doi T, Hiroaki Y, Arimoto I, Fujiyoshi Y, Okamoto T, Satoh M, Furuichi Y
Abstract excerpt
Endothelin type-B receptor (ET(B)R) forms a stable complex with its ligand, endothelin-1. To facilitate biochemical and biophysical studies of human ET(B)R, several ET(B)R mutants carrying a hexahistidine tag sequence at the N or C terminus were expressed in Sf9 cells and were purified by a combination of biotinylated endothelin-1-ligand-affinity and nickel-affinity chromatographies. The ligand-free receptor was...
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