Article
Expression of rat thrombin receptor and mutants in the baculovirus-infected insect cell system.
Thrombosis research - 15 Jul 1997
Henrikson K P, Quirk S M, Restivo A
Abstract excerpt
The rat thrombin receptor (TR-1) has been expressed in Sf9 cells. Two mutant receptors, one lacking a thrombin cleavage site (RTRM), and the other containing only the extracellular domain of the receptor (ECD), have been expressed. Antibodies to the thrombin receptor activating peptide (TRAP) and to the fibrinogen-binding exosite have been prepared. On Western blots these antibodies bound to TR-1 bands of 65-70...
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