Article
Characterization and application of monoclonal antibodies against human endothelin B receptor expressed in insect cells.
Biotechnology letters - 1 Feb 2004
Yamaguchi Tomohiro, Arimoto-Tahara Ikuyo, Fujiyoshi Yoshinori, Doi Tomoko
Abstract excerpt
Endothelin B receptor (ET(B)R) is a G protein-coupled receptor that mediates a variety of signals by binding to vasoconstrictive peptides, endothelins. Monoclonal antibodies were prepared against human ET(B)R using the full-length protein expressed in Sf9 cells. Five typical monoclonal antibodies were characterized further for their recognition. The epitopes for the 2A5, 9A3 and 21A1 antibodies were mapped within...
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