Article
A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor.
The EMBO journal - 1 Aug 1997
Preker P J, Ohnacker M, Minvielle-Sebastia L, Keller W
Abstract excerpt
Polyadenylation is the second step in 3' end formation of most eukaryotic mRNAs. In Saccharomyces cerevisiae, this step requires three trans-acting factors: poly(A) polymerase (Pap1p), cleavage factor I (CF I) and polyadenylation factor I (PF I). Here, we describe the purification and subunit com...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA Primers
- Evolution, Molecular
- Fungal Proteins
- Genes, Fungal
- Macromolecular Substances
- Molecular Sequence Data
- Multiprotein Complexes
- Mutation
- Polynucleotide Adenylyltransferase
- Protein Conformation
- RNA Processing, Post-Transcriptional