Article
Improved amplification system for detection of hepatitis C virus genome that simultaneously differentiates viral genotypes.
The Southeast Asian journal of tropical medicine and public health - 1 Jun 1996
Songsivilai S, Kanistanon D, Panyavinin W, Neelamek M, Dharakul T
Abstract excerpt
An improved system for amplification of hepatitis C virus genome (HCV) was developed based on a multiplex nested polymerase chain reaction format. Two sets of oligonucleotide primers were used simultaneously. One was derived from the conserved sequences in the 5' non-coding region of the viral ge...
Topics
- Blood Donors
- DNA Primers
- Genetic Variation
- Genome, Viral
- Genotype
- Hepacivirus
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- RNA, Viral
- Reproducibility of Results
- Sensitivity and Specificity