Article
Improved amplification system for detection of hepatitis C virus genome that simultaneously differentiates viral genotypes.
The Southeast Asian journal of tropical medicine and public health - 1 Jun 1996
Songsivilai S, Kanistanon D, Panyavinin W, Neelamek M, Dharakul T
Abstract excerpt
An improved system for amplification of hepatitis C virus genome (HCV) was developed based on a multiplex nested polymerase chain reaction format. Two sets of oligonucleotide primers were used simultaneously. One was derived from the conserved sequences in the 5' non-coding region of the viral genome which can bind to the viral genome of all genotypes. The other set of primers was designed from a sequence in the...
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