Article
[Establishing DNA chip technique for detecting hepatitis C virus genotypes and primary application].
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Dec 2003
Mao Hong-ju, Gu Shi-min, Zhao Jian-long, Liu Jiang-xia
Abstract excerpt
OBJECTIVE: To establish a new method based DNA chip technique for detecting HCV genotypes. METHODS: Genotyping probes were designed according to the sequence of HCV 5' NCR to generate DNA chip. The probes on DNA chip contains 5 major genotypes and 8 subtypes. The DNA fragment amplified by labeling Cy5 fluorescence was hybridized with DNA chip. RESULTS: Fifty-five out of 65 isolates detected by DNA chip belonged...
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