Article
[Hepatitis C virus genotyping by restriction fragment length polymorphism of polymerase chain reaction products generated with a HCV detection kit].
Rinsho byori. The Japanese journal of clinical pathology - 1 Jul 2001
Yukimasa N, Yoshida K, Ohkushi H, Tanabe S, Fukasawa K, Kanou M, Fukuchi K, Takagi Y, Gomi K
Abstract excerpt
Highly conserved sequence in the 5' untranslated region(UTR) of hepatitis C virus(HCV) genome have been targeted by most nucleic acid amplification-based detection assays, such as Amplicor HCV test, a commercially available assay kit. In this study, we classified HCV genotypes by direct sequencing determination for 5' UTR of nested-PCR after Amplicor HCV test. Then, based on the results of sequence, RFLP analysis...
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