Article
A simple PCR with different 3' ends of the third primer for detection of defined point mutations: HCV genotyping as an example.
Clinical chemistry and laboratory medicine - 1 Aug 1998
Alavantic D, Glisic S, Radovanović N, Romić M
Abstract excerpt
In this article, we describe a useful modification of the polymerase chain reaction for amplification applicable to hepatitis C virus genotyping and determination of its subtypes. The method is fast, cheap and simple for detection of any known point mutation, and could be used in every laboratory...
Topics
- Base Sequence
- DNA Primers
- Genotype
- Hepacivirus
- Humans
- Point Mutation
- Polymerase Chain Reaction
- RNA, Viral