Article
A simple PCR with different 3' ends of the third primer for detection of defined point mutations: HCV genotyping as an example.
Clinical chemistry and laboratory medicine - 1 Aug 1998
Alavantic D, Glisic S, Radovanović N, Romić M
Abstract excerpt
In this article, we describe a useful modification of the polymerase chain reaction for amplification applicable to hepatitis C virus genotyping and determination of its subtypes. The method is fast, cheap and simple for detection of any known point mutation, and could be used in every laboratory with experience in polymerase chain reaction technique. We could differentiate hepatitis C virus subtype 1b from other...
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