Article
Development of an improved genotyping assay for the detection of hepatitis C virus genotypes and subtypes in Pakistan.
Journal of virological methods - 1 Jun 2008
Idrees Muhammad
Abstract excerpt
A new genotyping system was established for the specific detection of HCV genotypes 1a, 1b, 1c, 2a, 2b, 2c, 3a, 3b, 3c, 4a-h, 5a and 6a during the course of this study. The system is based on entire core region and a part of 5' noncoding region (5'NCR) with genotype-specific primers. Genotype-specific primers were designed on the basis of 114 HCV isolates. Serum samples with known genotypes were used as positive...
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