Article
Double-strand breaks increase the incidence of genetic deletion associated with intermolecular recombination in bacteriophage T7.
Molecular & general genetics : MGG - 1 Jul 1997
Yang Y, Masker W
Abstract excerpt
An in vitro DNA replication system based on extracts prepared from Escherichia coli cells infected with bacteriophage T7 was used to study deletion associated with the repair of double-strand breaks. The gene for T7 ligase was interrupted by a DNA insert which included 17-bp direct repeats. Deletion between the repeats restored the reading frame of the gene, and these DNA molecules could be detected by their...
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