Article
Molecular mechanisms of deletion formation in Escherichia coli plasmids. I. Deletion formation mediated by long direct repeats.
Molecular & general genetics : MGG - 1 Aug 1991
Dianov G L, Kuzminov A V, Mazin A V, Salganik R I
Abstract excerpt
Derivatives of plasmid pBR327 with the tet gene interrupted by 165 pb or 401 bp direct repeats were constructed. In cells harboring these plasmids, deletions which restored the wild-type tet gene gave rise to tetracycline-resistant colonies, thereby allowing a simple phenotypic test for deletion...
Topics
- Chromosome Mapping
- Drug Resistance, Microbial
- Electrophoresis, Agar Gel
- Escherichia coli
- Gene Frequency
- Multigene Family
- Mutation
- Plasmids
- Polymorphism, Restriction Fragment Length
- Recombination, Genetic
- Repressor Proteins
- Tetracycline