Article
Structure and aminoacylation capacities of tRNA transcripts containing deoxyribonucleotides.
RNA (New York, N.Y.) - 1 Aug 1997
Aphasizhev R, Théobald-Dietrich A, Kostyuk D, Kochetkov S N, Kisselev L, Giegé R, Fasiolo F
Abstract excerpt
The contribution of the ribose 2'-hydroxyls to RNA structure and function has been analyzed, but still remains controversial. In this work, we report the use of a mutant T7 RNA polymerase as a tool in RNA studies, applied to the aspartate and methionine tRNA aminoacylation systems from yeast. Our...
Topics
- Base Sequence
- DNA-Directed RNA Polymerases
- Deoxyribonucleotides
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA, Transfer
- RNA, Transfer, Asp
- RNA, Transfer, Met
- Structure-Activity Relationship
- Transcription, Genetic
- Viral Proteins