Article
Efficient mischarging of a viral tRNA-like structure and aminoacylation of a minihelix containing a pseudoknot: histidinylation of turnip yellow mosaic virus RNA.
Nucleic acids research - 25 Apr 1992
Rudinger J, Florentz C, Dreher T, Giegé R
Abstract excerpt
Mischarging of the valine specific tRNA-like structure of turnip yellow mosaic virus (TYMV) RNA has been tested in the presence of purified arginyl-, aspartyl-, histidinyl-, and phenylalanyl-tRNA synthetases from bakers' yeast. Important mischarging of a 264 nucleotide-long transcript was found with histidinyl-tRNA synthetase which can acylate this fragment up to a level of 25% with a loss of specificity...
Topics
- Base Sequence
- Codon
- Histidine-tRNA Ligase
- Kinetics
- Molecular Sequence Data
- Mosaic Viruses
- Mutation
- Nucleic Acid Conformation
- RNA, Transfer, His
- RNA, Viral
- Yeasts