Article
Functional transfer RNAs with modifications in the 3'-CCA end: differential effects on aminoacylation and polypeptide synthesis.
Proceedings of the National Academy of Sciences of the United States of America - 25 Oct 1994
Liu M, Horowitz J
Abstract excerpt
The trinucleotide CCA sequence is present at the 3' terminus of all mature tRNAs. Despite this high degree of conservation, we have been able to prepare in vitro transcripts of Escherichia coli tRNA(Val) with altered 3' termini that are readily aminoacylated and can function in polypeptide synthesis. Replacement of the 3'-terminal adenosine with either cytidine or uridine yields a tRNA(Val) variant that retains...
Topics
- Base Sequence
- Cloning, Molecular
- Cytidine
- Escherichia coli
- Genetic Variation
- Kinetics
- Mutagenesis, Site-Directed
- Peptide Biosynthesis
- Protein Biosynthesis
- RNA, Transfer, Val
- Structure-Activity Relationship