C-terminal truncation of the transcriptional activator encoded by areA in Aspergillus nidulans results in both loss-of-function and gain-of-function phenotypes.

Abstract excerpt

Mutations truncating as many as 143 C-terminal residues from the transcriptional activator encoded by the areA gene, mediating nitrogen metabolite repression in Aspergillus nidulans, do not significantly reduce the ability of the areA product to activate expression of most genes under areA control. Such mutations can even have a gain-of-function, derepressed phenotype, consistent with a critical role for this...

Topics

• Amino Acid Sequence
• Aspergillus nidulans
• Base Sequence
• Fungal Proteins
• Gene Expression Regulation, Fungal
• Genes, Fungal
• Molecular Sequence Data
• Phenotype
• Sequence Deletion
• Transcription Factors