Article
Comparison of the protection of cells from antifolates by transduced human dihydrofolate reductase mutants.
Human gene therapy - 20 Nov 1997
Patel M, Sleep S E, Lewis W S, Spencer H T, Mareya S M, Sorrentino B P, Blakley R L
Abstract excerpt
Retroviral transduction of antifolate-resistant variants of human dihydrofolate reductase (hDHFR) into cells can increase their resistance to the cytotoxic effects of these drugs. We evaluated the ability of wild-type hDHFR and 20 mutant enzymes (13 with single-amino acid substitutions, 7 with tw...
Topics
- Aminopterin
- Animals
- Cell Survival
- Drug Resistance, Neoplasm
- Folic Acid Antagonists
- Gene Expression
- Genetic Variation
- Growth Inhibitors
- Humans
- Kinetics
- Methotrexate
- Pyrimidines
- Rabbits
- Tetrahydrofolate Dehydrogenase
- Thymidine
- Transfection
- Trimetrexate