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Article

Comparison of the protection of cells from antifolates by transduced human dihydrofolate reductase mutants.

Human gene therapy - 20 Nov 1997

Patel M, Sleep S E, Lewis W S, Spencer H T, Mareya S M, Sorrentino B P, Blakley R L

Abstract excerpt

Retroviral transduction of antifolate-resistant variants of human dihydrofolate reductase (hDHFR) into cells can increase their resistance to the cytotoxic effects of these drugs. We evaluated the ability of wild-type hDHFR and 20 mutant enzymes (13 with single-amino acid substitutions, 7 with two substitutions) to prevent growth inhibition in antifolate-treated CCRF-CEM cells. The wild-type enzyme and all of the...

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Identifiers

PMID: 9414255
DOI: 10.1089/hum.1997.8.17-2069

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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