Construction of a plasmid vector for transformation of Porphyromonas gingivalis.

doi:10.1111/j.1574-6968.1997.tb10425.x

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Article

Construction of a plasmid vector for transformation of Porphyromonas gingivalis.

FEMS microbiology letters - 1 Jul 1997

Yoshimoto H, Takahasi Y, Kato D, Umemoto T

Abstract excerpt

A host-vector system for transformation of Porphyromonas gingivalis was constructed using a set (1) strains that can incorporate plasmid DNA by electroporation regardless of its source and (2) stable vector plasmids with a selectable marker. First, restriction-negative mutants were isolated, beca...

Topics

Cloning, Molecular
Mutation
Plasmids
Porphyromonas gingivalis
Transformation, Bacterial

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