Article
Amino-terminal epitopes are exposed when full-length open reading frame 2 of hepatitis E virus is expressed inEscherichia coli, but carboxy-terminal epitopes are masked
1 Jul 1997
Abstract excerpt
We constructed a panel of overlapping and non-overlapping fragments of cDNA derived from open reading frame 2 (ORF2) of hepatitis E virus (HEV) and fused to the gene encoding glutathione S-transferase (GST), from which proteins were expressed in Escherichia coli. IgG-specific immunoreactivity against each protein was measured by Western immunoblotting using sera from experimentally infected Rhesus macaques...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
