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Article

Yeast cytosine deaminase mutants with increased thermostability impart sensitivity to 5-fluorocytosine.

Journal of molecular biology - 28 Mar 2008

Stolworthy Tiffany S, Korkegian Aaron M, Willmon Candice L, Ardiani Andressa, Cundiff Jennifer, Stoddard Barry L, Black Margaret E

Abstract excerpt

Prodrug gene therapy (PGT) is a treatment strategy in which tumor cells are transfected with a 'suicide' gene that encodes a metabolic enzyme capable of converting a nontoxic prodrug into a potent cytotoxin. One of the most promising PGT enzymes is cytosine deaminase (CD), a microbial salvage enzyme that converts cytosine to uracil. CD also converts 5-fluorocytosine (5FC) to 5-fluorouracil, an inhibitor of DNA...

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Identifiers

PMID: 18291415
DOI: 10.1016/j.jmb.2008.01.002
PMCID: PMC2330253

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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