Article
Differential effects of aromatic and charged residue substitutions in the RNA binding domains of the yeast poly(A)-binding protein.
Journal of molecular biology - 30 May 1997
Deardorff J A, Sachs A B
Abstract excerpt
The yeast poly(A)-binding protein (Pab1p) contains four RNA recognition motifs (RRMs). Site-directed mutations were introduced into each of these RRMs in order to investigate their relative contributions to specific and non-specific RNA binding, and to determine the consequences of these mutation...
Topics
- Amino Acid Sequence
- Amino Acids
- Binding Sites
- Conserved Sequence
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Oligoribonucleotides
- Poly(A)-Binding Proteins
- Polyribosomes
- RNA
- RNA-Binding Proteins
- Ribonucleoproteins
- Sequence Homology, Amino Acid
- Structure-Activity Relationship
- Substrate Specificity
- Yeasts