Article
Genetic analysis of second-site revertants of bacteriophage lambda integrase mutants.
Journal of bacteriology - 1 Jun 1997
Wu Z, Gumport R I, Gardner J F
Abstract excerpt
Bacteriophage lambda site-specific recombination is catalyzed by the phage-encoded integrase (Int) protein. Using a collection of 21 recombination-defective Int mutants, we performed a second-site reversion analysis. One of the primary mutants contained a valine-to-glutamic acid change at position 175 (V175E), and a pseudorevertant with a lysine change at this site (V175K) was also isolated. Relative to the...
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