Genetic analysis of second-site revertants of bacteriophage lambda integrase mutants.

doi:10.1128/jb.179.12.4030-4038.1997

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Article

Genetic analysis of second-site revertants of bacteriophage lambda integrase mutants.

Journal of bacteriology - 1 Jun 1997

Wu Z, Gumport R I, Gardner J F

Abstract excerpt

Bacteriophage lambda site-specific recombination is catalyzed by the phage-encoded integrase (Int) protein. Using a collection of 21 recombination-defective Int mutants, we performed a second-site reversion analysis. One of the primary mutants contained a valine-to-glutamic acid change at positio...

Topics

Bacteriophage lambda
Integrases
Mutation
Recombination, Genetic
Structure-Activity Relationship

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