Article
Complementation of bacteriophage lambda integrase mutants: evidence for an intersubunit active site.
The EMBO journal - 1 Dec 1993
Han Y W, Gumport R I, Gardner J F
Abstract excerpt
Site-specific recombination of bacteriophage lambda starts with the formation of higher-order protein--DNA complexes, called 'intasomes', and is followed by a series of steps, including the initial DNA cleavage, top-strand exchange, branch migration and bottom-strand exchange, to produce recombin...
Topics
- Bacteriophage lambda
- Binding Sites
- Catalysis
- Cloning, Molecular
- DNA Nucleotidyltransferases
- DNA, Viral
- Genetic Complementation Test
- Integrases
- Kinetics
- Mutagenesis
- Mutation
- Substrate Specificity