Article
Mutagenesis and homology modeling of the Tn21 integron integrase IntI1.
Biochemistry - 3 Mar 2009
Johansson Carolina, Boukharta Lars, Eriksson Jens, Aqvist Johan, Sundström Lars
Abstract excerpt
Horizontal DNA transfer between bacteria is widespread and a major cause of antibiotic resistance. For logistic reasons, single or combined genes are shuttled between vectors such as plasmids and bacterial chromosomes. Special elements termed integrons operate in such shuttling and are therefore vital for horizontal gene transfer. Shorter elements carrying genes, cassettes, are integrated in the integrons, or...
Topics
- Amino Acid Sequence
- Attachment Sites, Microbiological
- Autoradiography
- Biocatalysis
- DNA
- DNA Transposable Elements
- Electrophoresis, Polyacrylamide Gel
- Electrophoretic Mobility Shift Assay
- Hydrogen Bonding
- Integrases