Article
Analysis of insertion into secondary attachment sites by phage lambda and by int mutants with altered recombination specificity.
Journal of molecular biology - 20 Jun 2003
Rutkai Edit, Dorgai László, Sirot Regina, Yagil Ezra, Weisberg Robert A
Abstract excerpt
When phage lambda lysogenizes a cell that lacks the primary bacterial attachment site, integrase catalyzes insertion of the phage chromosome into one of many secondary sites. Here, we characterize the secondary sites that are preferred by wild-type lambda and by lambda int mutants with altered insertion specificity. The sequences of these secondary sites resembled that of the primary site: they contained two...
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