Near-simultaneous DNA cleavage by the subunits of the junction-resolving enzyme T4 endonuclease VII.

doi:10.1093/emboj/16.9.2528

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Article

Near-simultaneous DNA cleavage by the subunits of the junction-resolving enzyme T4 endonuclease VII.

The EMBO journal - 1 May 1997

Giraud-Panis M J, Lilley D M

Abstract excerpt

In common with a number of other DNA junction-resolving enzymes, endonuclease VII of bacteriophage T4 binds to a four-way DNA junction as a dimer, and cleaves two strands of the junction. We have used a supercoil-stabilized cruciform substrate to probe the simultaneity of cleavage at the two site...

Topics

Bacteriophage T4
DNA, Superhelical
DNA-Binding Proteins
Dimerization
Endodeoxyribonucleases
Genetic Complementation Test
Mutation
Nucleic Acid Conformation
Plasmids
Recombination, Genetic
Structure-Activity Relationship

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