Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Action of RecBCD enzyme on cruciform DNA.

Journal of molecular biology - 5 Jan 1990

Taylor A F, Smith G R

Abstract excerpt

We tested the hypothesis that RecBCD enzyme of Escherichia coli resolves pre-existing Holliday recombination intermediates by examining the action of the purified enzyme on an open-ended DNA cruciform with limited ability to branch migrate. The enzyme cleaved two strands of the cruciform near its base to produce "recombinant" products, with a marked bias in the direction of cleavage. The two nicks necessary to...

Topics

Base Sequence
DNA
Electrophoresis, Agar Gel
Electrophoresis, Gel, Two-Dimensional
Escherichia coli
Exodeoxyribonuclease V
Exodeoxyribonucleases
Genotype
Molecular Sequence Data
Nucleic Acid Conformation
Oligonucleotides

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

Repetitive Sequences, Nucleic Acid

Restriction Mapping

Substrate Specificity

Source

Identifiers

PMID: 2405161
DOI: 10.1016/0022-2836(90)90015-E

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2405161-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Action of RecBCD enzyme on cruciform DNA.PMID 2405161Read ↗

Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure.Semantic proximity Read ↗

Resolution of joint molecules by RuvABC and RecG following cleavage of the Escherichia coli chromosome by EcoKI.Semantic proximity Read ↗

RecG helicase promotes DNA double-strand break repair.Semantic proximity Read ↗

Genetic dissection of the biochemical activities of RecBCD enzyme.Semantic proximity Read ↗

Inverted repeats as genetic elements for promoting DNA inverted duplication: implications in gene amplification.Semantic proximity Read ↗

RecBCD enzyme overproduction impairs DNA repair and homologous recombination in Escherichia coli.Semantic proximity Read ↗

Chi hotspot activity in Escherichia coli without RecBCD exonuclease activity: implications for the mechanism of recombination.Semantic proximity Read ↗

Functions of multiple exonucleases are essential for cell viability, DNA repair and homologous recombination in recD mutants of Escherichia coli.Semantic proximity Read ↗

The nuclease domain of the Escherichia coli RecBCD enzyme catalyzes degradation of linear and circular single-stranded and double-stranded DNA.Semantic proximity Read ↗

Resolution of an early RecA-recombination intermediate by a junction-specific endonuclease.Semantic proximity Read ↗

Molecular mechanisms of Holliday junction processing in Escherichia coli.Semantic proximity Read ↗

Analysis of strand transfer and template switching mechanisms of DNA gap repair by homologous recombination in Escherichia coli: predominance of strand transfer.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.