Article
Action of RecBCD enzyme on cruciform DNA.
Journal of molecular biology - 5 Jan 1990
Taylor A F, Smith G R
Abstract excerpt
We tested the hypothesis that RecBCD enzyme of Escherichia coli resolves pre-existing Holliday recombination intermediates by examining the action of the purified enzyme on an open-ended DNA cruciform with limited ability to branch migrate. The enzyme cleaved two strands of the cruciform near its base to produce "recombinant" products, with a marked bias in the direction of cleavage. The two nicks necessary to...
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