Article
Combining the hok/sok, parDE, and pnd postsegregational killer loci to enhance plasmid stability.
Applied and environmental microbiology - 1 May 1997
Pecota D C, Kim C S, Wu K, Gerdes K, Wood T K
Abstract excerpt
To enhance plasmid segregational stability in bacterial cells, two pairs of independent postsegregational killing loci (genes which induce host killing upon plasmid loss) isolated from plasmids R1, R483, or RP4 (hok+/sok+ pnd+ or hok+/sok+ parDE+) were cloned into a common site of the beta-galact...
Topics
- Antisense Elements (Genetics)
- Bacterial Proteins
- Bacterial Toxins
- Cloning, Molecular
- DNA-Binding Proteins
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Lac Operon
- Nuclear Proteins
- Phenotype
- Plasmids
- Promoter Regions, Genetic
- RNA
- RNA, Bacterial
- Recombination, Genetic
- Restriction Mapping
- beta-Galactosidase