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Article

Combining the hok/sok, parDE, and pnd postsegregational killer loci to enhance plasmid stability.

Applied and environmental microbiology - 1 May 1997

Pecota D C, Kim C S, Wu K, Gerdes K, Wood T K

Abstract excerpt

To enhance plasmid segregational stability in bacterial cells, two pairs of independent postsegregational killing loci (genes which induce host killing upon plasmid loss) isolated from plasmids R1, R483, or RP4 (hok+/sok+ pnd+ or hok+/sok+ parDE+) were cloned into a common site of the beta-galactosidase expression vector pMJR1750 (ptac::lacZ+) to form a series of plasmids in which the effect of one or two...

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Identifiers

PMID: 9143123
DOI: 10.1128/aem.63.5.1917-1924.1997
PMCID: PMC168483

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