Article
Multicopy plasmid suppression of stationary phase chaperone toxicity in Escherichia coli by phosphogluconate dehydratase and the N-terminus of DnaK.
Molecular & general genetics : MGG - 15 Dec 1995
Rockabrand D, Blum P
Abstract excerpt
Overproduction of DnaK in Escherichia coli results in a bacteriocidal effect. This effect is most acute in stationary phase cells. A selection scheme was developed to isolate multicopy suppressors from an E. coli plasmid expression library, which overcome the stationary phase toxicity of excess D...
Topics
- Bacterial Proteins
- Cell Division
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- HSP70 Heat-Shock Proteins
- Hydro-Lyases
- Molecular Chaperones
- Mutation
- Plasmids
- Repressor Proteins