Article
Specific function of DNA ligase I in simian virus 40 DNA replication by human cell-free extracts is mediated by the amino-terminal non-catalytic domain.
The Journal of biological chemistry - 25 Apr 1997
Mackenney V J, Barnes D E, Lindahl T
Abstract excerpt
The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is believed to be due to DNA ligase I. This enzyme is composed of a 78-kDa carboxyl-terminal catalytic domain and a 24-kDa amino-terminal region that is not required for ligation activity in vitro. Extracts...
Topics
- Cell Line
- Cell-Free System
- DNA
- DNA Ligase ATP
- DNA Ligases
- DNA Replication
- DNA, Viral
- Genetic Complementation Test
- Humans
- Mutation
- Peptide Fragments
- Poly-ADP-Ribose Binding Proteins
- Recombinant Proteins
- Simian virus 40
- Xenopus Proteins