Article
In vivo ultraviolet and dimethyl sulfate footprinting of the 5' region of the expressed and silent Xist alleles.
The Journal of biological chemistry - 18 Apr 1997
Komura J i, Sheardown S A, Brockdorff N, Singer-Sam J, Riggs A D
Abstract excerpt
The Xist (X inactive specific transcript) gene plays an essential role in X chromosome inactivation. To elucidate the mechanisms controlling Xist expression and X inactivation, we examined in vivo DNA-protein interactions in the Xist promoter region in a female mouse cell line (BMSL2), which has...
Topics
- Alleles
- Animals
- Base Sequence
- Blotting, Southern
- Cell Line
- Crosses, Genetic
- DNA
- DNA Footprinting
- Female
- Liver
- Mice
- Mice, Inbred C57BL
- Mice, Mutant Strains
- Molecular Sequence Data
- Polymerase Chain Reaction
- Promoter Regions, Genetic
- RNA, Long Noncoding
- RNA, Untranslated