Article
The folded conformation of phage P22 coat protein is affected by amino acid substitutions that lead to a cold-sensitive phenotype.
Biochemistry - 1 Apr 1997
Fong D G, Doyle S M, Teschke C M
Abstract excerpt
Three cold-sensitive mutants in phage P22 coat protein have been characterized to determine the effects of the amino acid substitutions that cause cold sensitivity on the folding pathway and the conformation of refolded coat protein. Here we find that the three cold-sensitive mutants which have t...
Topics
- Anilino Naphthalenesulfonates
- Bacteriophage P22
- Capsid
- Chaperonin 10
- Chaperonin 60
- Chymotrypsin
- Electrophoresis, Polyacrylamide Gel
- Fluorescent Dyes
- Guanidine
- Guanidines
- Kinetics
- Mutation
- Protein Conformation
- Protein Denaturation
- Protein Folding
- Protein Structure, Secondary
- Salmonella typhimurium
- Serine Endopeptidases