Article
In vitro folding of phage P22 coat protein with amino acid substitutions that confer in vivo temperature sensitivity.
Biochemistry - 23 May 1995
Teschke C M, King J
Abstract excerpt
The coat protein that forms the icosahedral shell of phage P22 can be efficiently refolded in vitro [Teschke, C. M., & King, J. (1993) Biochemistry 32, 10839-10847]. Temperature-sensitive mutants of coat protein interfere with folding or assembly in vivo [Gordon, C. L., & King, J. (1993) J. Biol....
Topics
- Anilino Naphthalenesulfonates
- Bacteriophage P22
- Capsid
- Cross-Linking Reagents
- Electrophoresis, Polyacrylamide Gel
- Fluorescent Dyes
- Kinetics
- Macromolecular Substances
- Microscopy, Electron
- Mutation
- Protein Conformation
- Protein Denaturation
- Protein Folding
- Spectrometry, Fluorescence
- Succinimides
- Temperature