Characterization of a mutant recombinant S100 protein using electrospray ionization mass spectrometry.

doi:10.1002/(SICI)1097-0231(19970228)11:4<405::AID-RCM823>3.0.CO;2-A

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Characterization of a mutant recombinant S100 protein using electrospray ionization mass spectrometry.

Rapid communications in mass spectrometry : RCM - 1 Jan 1997

Raftery M J, Harrison C A, Geczy C L

Abstract excerpt

Two recombinant proteins derived by thrombin cleavage of a fusion protein between glutathione-S-transferase and CP10 (Chemotactic protein 10 kDa) were separated by C4 reversed-phase high-performance liquid chromatography (RP-HPLC). Both proteins were recognised by a polyclonal antibody to native CP10 following sodium dodecyl sulphate/polyacryamide gel electrophoresis (SDS/PAGE) and Western blotting. The major...

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Characterization of a mutant recombinant S100 protein using electrospray ionization mass spectrometry.

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