Article
Evaluation of mass spectrometric techniques for characterization of engineered proteins.
Molecular biotechnology - 1 Aug 1995
Roepstorff P, Schram K H, Andersen J S, Rafn K, Baldursson T, Krøll J, Poulsen K, Knudsen J, Kristiansen K
Abstract excerpt
Mass spectrometric characterization of engineered proteins has been examined using bovine recombinant Acyl-CoA-Binding Protein (rACBP), [15N]-labeled rACBP, and a number of sequence variants of ACBP produced by site-directed mutagenesis. The mass spectrometric techniques include ESIMS and MALDIMS for analysis of the intact protein. Peptide maps have been obtained either by direct analysis of enzymatically derived...
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