Allele walking: a new and highly accurate approach to HLA-DRB1 typing. Application to DRB1*04 alleles.

Abstract excerpt

We have developed a typing method, which can be used even in small laboratories, to produce a highly accurate and reliable allele assignment in any homozygous or heterozygous situation. We have called the method allele walking (AW) and it consists of sequential rounds of PCR-RFLP. After digestion, electrophoresis separates alleles positive for the mutation from the negative alleles; the cleaved fragment is then...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Alleles
• Share to Chromosome Walking
• Share to DNA