Article
New protease mutants in Aspergillus niger result in strongly reduced in vitro degradation of target proteins; genetical and biochemical characterization of seven complementation groups.
Current genetics - 1 Sept 1995
van den Hombergh J P, van de Vondervoort P J, van der Heijden N C, Visser J
Abstract excerpt
Several mutants of Aspergillus niger, deficient in extracellular protease expression, have been isolated and characterized both genetically and biochemically. The mutant strains, obtained after in vivo UV-mutagenesis of conidiospores and selected by haloscreening on a new dual-substrate plate ass...
Topics
- Aspergillus niger
- Electrophoresis, Polyacrylamide Gel
- Endopeptidases
- Fungal Proteins
- Genes, Fungal
- Genetic Complementation Test
- Genetic Linkage
- Hydrogen-Ion Concentration
- Mutagenesis
- Pepstatins
- Phenotype
- Protease Inhibitors
- Proteins
- Recombination, Genetic
- Serum Albumin, Bovine
- Tosyl Compounds