Metabolic engineering of a non-allosteric citrate synthase in an Escherichia coli citrate synthase mutant.

doi:10.1002/jmr.300080602

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Article

Metabolic engineering of a non-allosteric citrate synthase in an Escherichia coli citrate synthase mutant.

Journal of molecular recognition : JMR - 1 Jan 2000

Evans C T

Abstract excerpt

This study examined the organization of the Krebs tricarboxylic acid (TCA) cycle by metabolic engineering and high-resolution 13C NMR. The oxidation of [1,2,3-13C]propionate to glutamate via the TCA cycle was measured in wild-type (WT) and a citrate synthase mutant (CS-) strain of Escherichia col...

Topics

Allosteric Regulation
Allosteric Site
Animals
Bacterial Proteins
Citrate (si)-Synthase
Escherichia coli
Magnetic Resonance Spectroscopy
Mutation
Oxidation-Reduction
Propionates
Swine

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