Article
Metabolic engineering of a non-allosteric citrate synthase in an Escherichia coli citrate synthase mutant.
Journal of molecular recognition : JMR - 1 Jan 2000
Evans C T
Abstract excerpt
This study examined the organization of the Krebs tricarboxylic acid (TCA) cycle by metabolic engineering and high-resolution 13C NMR. The oxidation of [1,2,3-13C]propionate to glutamate via the TCA cycle was measured in wild-type (WT) and a citrate synthase mutant (CS-) strain of Escherichia coli transformed with allosteric E. coli citrate synthase (ECCS) or non-allosteric pig citrate synthase (PCS). The 13C...
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