Article
Purification and analysis of an extremely halophilic beta-galactosidase from Haloferax alicantei.
Biochimica et biophysica acta - 8 Feb 1997
Holmes M L, Scopes R K, Moritz R L, Simpson R J, Englert C, Pfeifer F, Dyall-Smith M L
Abstract excerpt
As a first step in the development of a reporter system for gene expression in halophilic archaea, a beta-galactosidase was purified 140-fold from Haloferax alicantei (previously phenon K, strain Aa2.2). An overproducing mutant was first isolated by UV mutagenesis and screening on agar plates containing X-Gal substrate. Cytoplasmic extracts of the mutant contained 25-fold higher enzyme levels than the parent....
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