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Article

Efficient and rapid purification of recombinant human alpha-galactosidase A by affinity column chromatography.

Protein expression and purification - 1 Oct 2004

Yasuda Kayo, Chang Hui-Hwa, Wu Hui-Li, Ishii Satoshi, Fan Jian-Qiang

Abstract excerpt

The lysosomal enzyme alpha-galactosidase A (alpha-Gal A) metabolizes neutral glycosphingolipids that possess alpha-galactoside residues at the non-reducing terminus, and inherited defects in the activity of alpha-Gal A lead to Fabry disease. We describe here an efficient and rapid purification procedure for recombinant alpha-Gal A by sequential Concanavalin A (Con A)-Sepharose and immobilized...

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Identifiers

PMID: 15358377
DOI: 10.1016/j.pep.2004.07.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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