Article
Isolation and characterization of a new family 42 beta-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: identification of the active site residues.
Biochimica et biophysica acta - 1 Feb 2008
Di Lauro Barbara, Strazzulli Andrea, Perugino Giuseppe, La Cara Francesco, Bedini Emiliano, Corsaro Maria Michela, Rossi Mosè, Moracci Marco
Abstract excerpt
The thermoacidophilic bacterium Alicyclobacillus acidocaldarius is a rich source of glycoside hydrolases enabling its growth on several di- and polysaccharides. We report here the purification and the characterization of a beta-galactosidase from this source, the cloning of its gene, and the expression and the characterization of the recombinant enzyme (Aabeta-gal). The enzyme was purified 46-fold from A....
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