Article
Rapid and sensitive genotyping of hepatitis C virus by single-strand conformation polymorphism.
Journal of virological methods - 1 Feb 1997
Lareu R R, Swanson N R, Fox S A
Abstract excerpt
There is an increasing demand for genotyping hepatitis C virus (HCV) isolates due to the rapidly expanding list of distinct HCV genotypes and the mounting evidence of genotype-specific clinical consequences. We describe an SSCP-based assay for determining genotypes in HCV infections. HCV RNA extracted from serum was amplified by a sensitive nested-PCR assay producing a 287 bp fragment of the conserved 5'...
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