Article
Genotyping of human cytomegalovirus using non-radioactive single-strand conformation polymorphism (SSCP) analysis.
Journal of virological methods - 9 Jun 2003
de Albuquerque Dulcinéia Martins, Costa Sandra Cecília Botelho
Abstract excerpt
Genetic variation in the glycoprotein B (gB) gene may play a role in human cytomegaloviruses (HCMVs) pathogenesis. Using restriction analysis of the gB gene product (PCR-RFLP), amplified by the nested polymerase chain reaction, the HCMV strains can be compared and classified into at least four HCMV groups. PCR single-strand conformation polymorphism (PCR-SSCP) is one of the techniques used to identify a mutant...
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